Pettenkofer Test. Pettenkofer’s Test for bile in urine etc Put a small quantity of the suspected liquid into a testtube and add to it drop by drop strong sulphuric acid till it becomes warm taking care not to raise the temperature above 122° Fabr Then add from 2 to 5 drops of syrup made with 5 parts of sugar to 4 of water and shake the mixture.
The Pettenkofer (31) test is probably the most sensitive reaction known at present for the bile acids As such it offers the best basis for a method which can be readily adapted for use with smal1 amounts of blood.
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TheorySmith’s TestPettenkofer’S TestMaterial Required1 Test tube 2 Spatula 3 Dropper 4 Sucrose 5 Urine sample 6 Measuring cylinder 7 Concentrated H2SO4Procedure1 Take a clean and dried test tube 2 Add accurately add 2ml of urine sample into the test tube using a measuring cylinder 3 With the help of a spatula add some sucrose into the tube holding the urine sample 4 With the help of a measuring cylinder accurately measure 2ml of concentra.
STUDIES IN THE METABOLISM OF THE BILE: I. A QUANTITATIVE
Pettenkofer Test November 04 2013 Objective This test is used to prove the presence of bile salt Base Theory Bile salt will reacts with hydroxymethylenephurphural to forms red solution Hydroxymethylenephurphural is formed of.
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Pettenkofers Test Pettenkofer’s Test for the presence of bile salts consists in the addition to the suspected liquid of a drop of a strong solution of cane sugar and then of sulphuric acid If bile salts are present a bright cherryred colour is developed.
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PETTENKOFER’S TEST Objective This test is used to prove the presence of bile salt Base Theory Bile salt will reacts with hydroxymethylenephurphural to forms red solution Hydroxymethylenephurphural is formed of sugar that dehidrated with sulphuric acid Procedure Dissolve few crystals of saccharose in 1 ml of diluted bile salt solution.